How To Immortalize Primary Cells For Creating A Cell Line?

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Aug 8, 2025
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Immortalizing primary cells is a key technique in modern research to develop stable and reproducible cell lines. Primary cells, by nature, have a limited lifespan and undergo senescence after a few passages. To overcome this, scientists introduce genes such as hTERT (human telomerase reverse transcriptase) or SV40 large T antigen through viral transfection or gene-editing methods. These genes help the cells bypass normal aging processes, allowing them to proliferate indefinitely while maintaining their original characteristics.
Before immortalization, it’s essential to start with high-quality, ethically sourced primary cells to ensure reliability in results. At Kosheeka, researchers can access a wide range of well-characterized human and animal primary cells with high viability and purity. Post-immortalization, cell lines are validated for morphology, genetic stability, and marker expression to confirm authenticity and performance.
By combining reliable primary cell sources from Kosheeka with advanced immortalization methods, scientists can create long-term, consistent models for drug testing, disease research, and regenerative medicine — accelerating breakthroughs in biomedical science.

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